Contents of Kit

A. Kit Materials Provided:
The 4A4 anti-p63 monoclonal antibody is provided at a working dilution in a buffer containing 50 mM Tris-HCl, pH 7.2, and 15 mM sodium azide.
Antigen retrival buffer: 50mM citrate buffer pH 6.0.
Wash buffer containing phosphate-buffered saline (PBS).
Secondary antibody containing working dilution of goat anti-mouse immunoglobulin conjugated to horseradish peroxidase (HRP) in a buffer containing 50 mM Tris-HCl, pH 7.2 and 0.2mM EDTA.
HRP visualization kit.

B. Kit Materials required, but not provided:
Counterstains: hematoxylin, eosin.
Coverslips.
Distilled or deionized water.
Drying oven, capable of maintaining 60oC.
Epitope retrieval solution (see Specimen Preparation; pretreatment of tissue).
Ethanol (absolute and 95%).
Light Microscope (4-40X objective magnification).
Microscope slides (SuperFrost Plus, poly-L-lysine.
Microwave oven (750 watt).
Mounting medium.
Staining jars or baths
Timer (capable of 2-40 min intervals).
Wash Buffer.
Water bath with lid (capable of maintaining epitope retrieval solution at 95-99oC).
Xylene, toluene, or xylene substitutes.

Precautions & Warnings
1. For professional users only.
2. This product contains sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing.
3. As with any product derived from biological sources, proper handling procedures should be used.
4. For In Vitro Diagnostic Use.

Storage:
Store at 2-8ºC. Prostate-63 Cancer Diagnostic Test is suitable for use 6 months from the date of manufacture when stored at 2-8ºC. Do not use after expiration date present on vial/package. If reagents are stored under any conditions other than those specified, the user must verify the conditions. There are no obvious signs to indicate instability of this product. Therefore, positive and negative controls should be run simultaneously with patient specimens. If unexpected staining is observed which cannot be explained by variations in laboratory procedures and a problem with the antibody is suspected, contact our Technical Services.